AB0062 TIME COURSE OF INTESTINAL PERMEABILITY AND BACTERIAL TRANSLOCATION IN THE MODEL OF ADJUVANT-INDUCED ARTHRITIS

Background: Intestinal inflammation, dysbiosis, intestinal permeability (IP) and bacterial translocation (BT) have been identified in patients with spondyloarthritis but the time at which they appear their contribution to pathogenesis of disease is still a matter debate. Objectives: To investigate time-course IP BT rat model reactive arthritis, subgroup SpA, adjuvant-induced arthritis model. Methods: Adjuvant-induced (AIA) was induced 6-week-old male Lewis rats by an injection base tail Mycobacterium butyricum incomplete Freund’s adjuvant (Day (D) 0). Control received saline using same procedure. Body weights clinical score were daily assessed. A group AIA control (n=15 per group) euthanized three different times arthritis: D4 for pre-arthritic phase (AIA-preclinical), D11 onset (AIA-onset) D28 acute (AIA-acute). In each (AIA control, n=15 group)), assessed measuring plasma levels zonulin (ELISA) ileal mRNA expression occludin (RT-qPCR), studied endotoxins (or LPS, LCMS 2 method), soluble CD-14 (sCD14, ELISA) TLR-4, inflammation IL-8, IL-33, IL-17, IL-23p19 TNF-α (RT-qPCR). Joint damage determination radiographic hind paws. Results: decreased from as compared controls, parallel development severe arthritic D28. Compared rats, increase D4, not Ileal overexpression occurred while unchanged. As early Day 4 (preclinical phase), IL-33 IL-17 overexpressed ileum AIA. At 11 (onset), IL-8 persisted increased Neither LPS nor TLR-4 changed whatever arthritis. By contrast, blood sCD-14 significantly all stages No correlation found between scores or levels. Conversely, negative observed (r=-0.3, p=0.02). Conclusion: animal occur prior joint suggesting role these disorders this disease. Acknowledgements: I would like thank Société Française de Rhumatologie its support work. Disclosure Interests: None declared